{"id":75,"date":"2011-06-17T14:26:36","date_gmt":"2011-06-17T05:26:36","guid":{"rendered":"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/?p=75"},"modified":"2014-02-10T09:37:10","modified_gmt":"2014-02-10T00:37:10","slug":"mmessage_mmachine_high_yield_c","status":"publish","type":"post","link":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/?p=75","title":{"rendered":"mMESSAGE mMACHINE\u00ae High Yield Capped RNA Transcription Kit"},"content":{"rendered":"<p>\u30ad\u30e3\u30c3\u30d7\u69cb\u9020\u3092\u6301\u3064RNA\u3092\u5927\u91cf\u306b\u5897\u5e45\u3067\u304d\u308bmMESSAGE mMACHINE\u306b\u306f\u3001Ambion\u304c\u7279\u8a31\u3092\u6301\u3064\u5927\u91cf\u5897\u5e45\u306e\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u3092\u53d6\u308a\u5165\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u306b\u3088\u308a\u30012\u6642\u9593\u306e\u53cd\u5fdc\u306720\u00b5l\u306e\u91cf\u306e1\u00b5g\u306e\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8DNA\u304b\u3089\u30017-methyl guanosine\u306e\u30ad\u30e3\u30c3\u30d7\u69cb\u9020\u3092\u6301\u306415\uff5e35\u00b5g \u306eRNA\u3092\u5408\u6210\u3067\u304d\u307e\u3059\u3002\u3053\u306e\u91cf\u306f\u3001\u5f93\u6765\u306ein vitro\u8ee2\u5199\u53cd\u5fdc\u3067\u5f97\u3089\u308c\u308b\u30ad\u30e3\u30c3\u30d7\u69cb\u9020\u3092\u6301\u3064RNA\u91cf\u306e10\uff5e50\u500d\u306b\u76f8\u5f53\u3057\u307e\u3059\u3002\u53ce\u91cf\uff0815\uff5e35\u00b5g\uff09\u3068\u30ad\u30e3\u30c3\u30d7\u69cb\u9020\u3092\u6301\u3064\u8ee2\u5199\u7523\u7269\u306e\u5272\u5408\uff0880%\uff09\u306e\u30d0\u30e9\u30f3\u30b9\u304c\u6700\u9069\u306b\u306a\u308b\u3088\u3046\u306b\u3001GTP\u306b\u5bfe\u3059\u308b\u30ad\u30e3\u30c3\u30d7\u30a2\u30ca\u30ed\u30b0\u306e\u5272\u5408\u3092\u81f3\u9069\u5316\u3057\u3066\u3042\u308a\u307e\u3059\u3002mMESSAGE mMACHINE kit\u306b\u306f\u3001\u6c88\u6bbf\u7528\u306eLiCl\u6eb6\u6db2\u304c\u5165\u3063\u3066\u3044\u308b\u306e\u3067\u3001\u30ad\u30e3\u30c3\u30d7\u69cb\u9020\u3092\u6301\u3064RNA\u304b\u3089\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u672a\u53cd\u5fdc\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\uff08\u30ad\u30e3\u30c3\u30d7\u30a2\u30ca\u30ed\u30b0\u3092\u542b\u3080\uff09\u3092\u52b9\u679c\u7684\u306b\u5206\u96e2\u3067\u304d\u3001\u7ffb\u8a33\u306e\u52b9\u7387\u304c\u4e0a\u304c\u308a\u307e\u3059\u3002\u305d\u308c\u305e\u308c\u306emMESSAGE mMACHINE kit\u306b\u306f38 A260 Units (SP6) \u300157A260 Units (T7\u3068T3) \u306e\u30ad\u30e3\u30c3\u30d7\u30a2\u30ca\u30ed\u30b0\u304c\u5165\u3063\u3066\u304a\u308a\u300125\u56de\u306e\u53cd\u5fdc\u304c\u53ef\u80fd\u3067\u3059\u3002\u9577\u3044\u8ee2\u5199\u7523\u7269\uff08>4kb\uff09\u306e\u53ce\u91cf\u3092\u5897\u52a0\u3055\u305b\u308b\u305f\u3081\u306b\u3001\u30ad\u30c3\u30c8\u306b\u5165\u3063\u3066\u3044\u308bGTP stock solution\u3092\u52a0\u3048\u3066\u30ad\u30e3\u30c3\u30d7\u30a2\u30ca\u30ed\u30b0\u3068GTP\u306e\u6bd4\u3092\u8abf\u6574\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002mMESSAGE mMACHINE\u3092\u7528\u3044\u3066\u5408\u6210\u3055\u308c\u305fRNA\u306f\u30de\u30a4\u30af\u30ed\u30a4\u30f3\u30b8\u30a7\u30af\u30b7\u30e7\u30f3\u3084in vitro\u7ffb\u8a33\u306b\u6700\u9069\u3067\u3059\u3002<br \/>\n<a href=\"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/fm_1340.pdf\">\u30de\u30cb\u30e5\u30a2\u30eb<\/a><br \/>\n<a href=\"http:\/\/www.ambion.com\/jp\/catalog\/ProdGrp.html?fkApp=10&#038;fkProdGrp=99\">\u88fd\u54c1\u30da\u30fc\u30b8<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u30ad\u30e3\u30c3\u30d7\u69cb\u9020\u3092\u6301\u3064RNA\u3092\u5927\u91cf\u306b\u5897\u5e45\u3067\u304d\u308bmMESSAGE 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