{"id":72,"date":"2011-04-21T12:47:54","date_gmt":"2011-04-21T03:47:54","guid":{"rendered":"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/?p=72"},"modified":"2014-02-10T09:37:10","modified_gmt":"2014-02-10T00:37:10","slug":"toyobo_rtaq","status":"publish","type":"post","link":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/?p=72","title":{"rendered":"TOYOBO rTaq"},"content":{"rendered":"<p>5 \u00b5l 10 Buffer<br \/>\n5 \u00b5l 2mM dNTPs<br \/>\n3 \u00b5l MgCl2<br \/>\n1 \u00b5l 10 \u00b5M primers<br \/>\nx \u00b5l template DNA (*)<br \/>\n0.5-1 \u00b5l 2.5U\/l rTaq<br \/>\nH2O to 50 \u00b5l<br \/>\n* Genomic DNA: 10-1000 ng, plasmid DNA: 1-50 ng, cDNA: -200 ng<br \/>\n94\u02daC, 2 min<br \/>\n\u2193<br \/>\n25-40x :<br \/>\n94\u02daC, 30 sec<br \/>\n(Tm &#8211; 5) \u02daC, 30 sec<br \/>\n72\u02daC, 1 min\/kb<br \/>\n<a href=\"http:\/\/www.toyobo.co.jp\/e\/seihin\/xr\/lifescience\/products\/pcr_007.html\">http:\/\/www.toyobo.co.jp\/e\/seihin\/xr\/lifescience\/products\/pcr_007.html<\/a><br \/>\n\u6dfb\u4ed8\u6587\u66f8\u3084\u65e5\u672c\u8a9e\u30b5\u30a4\u30c8\u306b\u306f\u30d7\u30ed\u30c8\u30b3\u30eb\u304c\u306a\u304f\u3001\u82f1\u8a9e\u30b5\u30a4\u30c8\u306b\u7c21\u5358\u306a\u306e\u304c\u3042\u308b\u3060\u3051\u3002\u3082\u3046\u8cb7\u308f\u306a\u3044\u307b\u3046\u304c\u3044\u3044<\/p>\n","protected":false},"excerpt":{"rendered":"<p>5 \u00b5l 10 Buffer 5 \u00b5l 2mM dNTPs 3 \u00b5l MgCl2 1 \u00b5l 10 \u00b5M primers x \u00b5l template DNA (*) 0.5-1 \u00b5l 2.5U\/l rTaq H2O to <\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":false,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[4],"tags":[],"class_list":["post-72","post","type-post","status-publish","format-standard","hentry","category-molecular-biology"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p4fFyn-1a","jetpack-related-posts":[],"_links":{"self":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/72","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=72"}],"version-history":[{"count":1,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/72\/revisions"}],"predecessor-version":[{"id":185,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/72\/revisions\/185"}],"wp:attachment":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=72"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=72"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=72"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}