{"id":62,"date":"2006-09-07T14:59:04","date_gmt":"2006-09-07T05:59:04","guid":{"rendered":"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/?p=62"},"modified":"2014-02-10T09:39:39","modified_gmt":"2014-02-10T00:39:39","slug":"takara_primestar_hs","status":"publish","type":"post","link":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/?p=62","title":{"rendered":"TaKaRa PrimeSTAR HS"},"content":{"rendered":"<p>\u9ad8\u3044\u6b63\u78ba\u6027\u3068\u9ad8\u3044\u5897\u5e45\u7387\u3092\u4f75\u305b\u6301\u3064PCR\u9175\u7d20<br \/>\n\u5e38\u6e29\u4e0b\u3067\u306eDNA polymerase\u6d3b\u6027\u30fbexonuclease\u6d3b\u6027\u3092\u6291\u3048\u308b\u6297\u4f53\u3092\u542b\u3080<br \/>\n\u5e73\u6ed1\u672b\u7aef<br \/>\n10 \u03bcl <strong><u>5x<\/u><\/strong> PrimeSTAR Buffer<br \/>\n4 \u03bcl 2.5 mM dNTP Mixture<br \/>\n0.2 &#8211; 0.3 \u03bcM Primer 1<br \/>\n0.2 &#8211; 0.3 \u03bcM Primer 2<br \/>\n&lt; 200 ng Template<br \/>\n0.5 \u03bcl PrimeSTAR HS DNA Polymerase (2.5 u\/\u03bcl)<br \/>\nH2O to 50 \u03bcl<br \/>\n30 cycles:<br \/>\n98\u2103 10 sec<br \/>\n55\u2103 <strong><u>5 sec<\/u><\/strong> (15 sec)<br \/>\n72\u2103 1 min\/kbp<br \/>\n<a href=\"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/PrimeSTAR%20HS.pdf\">\u30de\u30cb\u30e5\u30a2\u30eb<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u9ad8\u3044\u6b63\u78ba\u6027\u3068\u9ad8\u3044\u5897\u5e45\u7387\u3092\u4f75\u305b\u6301\u3064PCR\u9175\u7d20 \u5e38\u6e29\u4e0b\u3067\u306eDNA polymerase\u6d3b\u6027\u30fbexonuclease\u6d3b\u6027\u3092\u6291\u3048\u308b\u6297\u4f53\u3092\u542b\u3080 \u5e73\u6ed1\u672b\u7aef 10 \u03bcl 5x PrimeSTAR Buffer 4 \u03bcl 2.5 mM <\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":false,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[4],"tags":[],"class_list":["post-62","post","type-post","status-publish","format-standard","hentry","category-molecular-biology"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p4fFyn-10","jetpack-related-posts":[],"_links":{"self":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/62","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=62"}],"version-history":[{"count":1,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/62\/revisions"}],"predecessor-version":[{"id":230,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/62\/revisions\/230"}],"wp:attachment":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=62"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=62"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=62"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}