{"id":61,"date":"2006-09-09T23:15:12","date_gmt":"2006-09-09T14:15:12","guid":{"rendered":"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/?p=61"},"modified":"2014-02-10T09:39:39","modified_gmt":"2014-02-10T00:39:39","slug":"microinjection_buffer","status":"publish","type":"post","link":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/?p=61","title":{"rendered":"Microinjection buffer"},"content":{"rendered":"<div>\n\u30bc\u30d6\u30e9\u30d5\u30a3\u30c3\u30b7\u30e5\u3067\u306e\u5f37\u5236\u767a\u73fe\u306e\u305f\u3081RNA\u3084DNA\u3092\u30de\u30a4\u30af\u30ed\u30a4\u30f3\u30b8\u30a7\u30af\u30b7\u30e7\u30f3\u3059\u308b\u3068\u304d\u306b\u7528\u3044\u308b\n<\/div>\n<ul>\n<li>0.25% phenol red<\/li>\n<li>140 mM KCl<\/li>\n<li>1 mM MgCl<\/li>\n<li>8 mM NaCl<\/li>\n<li>10 mM Hepes, pH adjusted to 7.2 with KOH<\/li>\n<\/ul>\n<div>\n2\u00d7\u301c4\u00d7\u6fc3\u5ea6\u3067\u8abf\u88fd\u3057\u3066\u30aa\u30fc\u30c8\u30af\u30ec\u30fc\u30d6\u3057\u3001\u5206\u6ce8\u3057\u3066\u51b7\u8535\u4fdd\u5b58\u3059\u308b\u3002<a href=\"http:\/\/www.biochemj.org\/bj\/332\/0005\/3320005.pdf\">intracellular buffer<\/a>\u306b\u57fa\u3065\u304f\u3002PBS\u3084TE\u3092\u30a4\u30f3\u30b8\u30a7\u30af\u30b7\u30e7\u30f3\u3057\u305f\u5834\u5408\u3088\u308a\u751f\u5b58\u7387\u304c\u3088\u3044\u3088\u3046\u3060\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u30bc\u30d6\u30e9\u30d5\u30a3\u30c3\u30b7\u30e5\u3067\u306e\u5f37\u5236\u767a\u73fe\u306e\u305f\u3081RNA\u3084DNA\u3092\u30de\u30a4\u30af\u30ed\u30a4\u30f3\u30b8\u30a7\u30af\u30b7\u30e7\u30f3\u3059\u308b\u3068\u304d\u306b\u7528\u3044\u308b 0.25% phenol red 140 mM KCl 1 mM MgCl 8 mM NaCl 10 mM Hepes, pH <\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":false,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[3,9],"tags":[],"class_list":["post-61","post","type-post","status-publish","format-standard","hentry","category-buffers-media","category-developmental-biology"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p4fFyn-Z","jetpack-related-posts":[],"_links":{"self":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/61","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=61"}],"version-history":[{"count":1,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/61\/revisions"}],"predecessor-version":[{"id":229,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/61\/revisions\/229"}],"wp:attachment":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=61"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=61"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=61"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}