{"id":45,"date":"2006-09-13T16:23:28","date_gmt":"2006-09-13T07:23:28","guid":{"rendered":"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/?p=45"},"modified":"2014-02-10T09:39:15","modified_gmt":"2014-02-10T00:39:15","slug":"bigdye18","status":"publish","type":"post","link":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/?p=45","title":{"rendered":"BigDye\u30921\/8\u306b\u7bc0\u7d04\u3059\u308b"},"content":{"rendered":"<ul>\n<li>1 \u03bcl BigDye Terminator Ready Reaction Mix<\/li>\n<li>1.5 \u03bcl 5\u00d7 Sequencing buffer<\/li>\n<li>0.8 pmol primer<\/li>\n<li>50\u301c120 ng template dsDNA<\/li>\n<li>H2O to 10 \u03bcl<\/li>\n<\/ul>\n<p><!--more--><\/p>\n<h4>5\u00d7 Sequencing buffer<\/h4>\n<ul>\n<li>10 mM MgCl2<\/li>\n<li>400 mM Tris-HCl, pH9.0<\/li>\n<\/ul>\n<h4>\u7cbe\u88fd<\/h4>\n<ol>\n<li>\u53cd\u5fdc\u304c\u7d42\u308f\u3063\u305f\u3089\u30011.5 ml\u30c1\u30e5\u30fc\u30d6\u306b\u79fb\u3057\u3001\u4ee5\u4e0b\u3092\u52a0\u3048\u308b<\/li>\n<ul>\n<li>1 \u03bcl 3M NaOAc, pH 4.6<\/li>\n<li>25 \u03bcl 95% EtOH<\/li>\n<\/ul>\n<li>\u6df7\u548c\u3057\u3001\u5ba4\u6e29\u306715\u5206\u9593\u9759\u7f6e<\/li>\n<li>2000-3000 \u00d7 g\u306730\u5206\u9593\u9060\u5fc3<\/li>\n<li>\u30a2\u30b9\u30d4\u30ec\u30fc\u30bf\u30fc\u3067\u4e0a\u6f84\u307f\u3092\u9664\u304f<\/li>\n<li>75 \u03bcl 70% EtOH\u3092\u52a0\u3048\u3001\u6570\u56de\u8ee2\u5012\u6df7\u548c<\/li>\n<li>2000-3000 \u00d7 g\u306730\u5206\u9593\u9060\u5fc3<\/li>\n<li>\u30a2\u30b9\u30d4\u30ec\u30fc\u30bf\u30fc\u3067\u4e0a\u6f84\u307f\u3092\u9664\u304f<\/li>\n<li>\u98a8\u4e7e\uff08\u4e7e\u304b\u3057\u3059\u304e\u306a\u3044\u3053\u3068\uff09<\/li>\n<li>10 \u03bcl\u306e\u6cf3\u52d5\u7528\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u52a0\u3048\u3066\u6eb6\u304b\u3059<\/li>\n<\/ol>\n<div>\n<a href=\"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/BigDye%20original%20and%20ver2.pdf\">BigDye\u306e\u30de\u30cb\u30e5\u30a2\u30eb<\/a>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>1 \u03bcl BigDye Terminator Ready Reaction Mix 1.5 \u03bcl 5\u00d7 Sequencing buffer 0.8 pmol primer 50\u301c120 ng template dsDNA<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":false,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[4],"tags":[],"class_list":["post-45","post","type-post","status-publish","format-standard","hentry","category-molecular-biology"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/s4fFyn-bigdye18","jetpack-related-posts":[],"_links":{"self":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/45","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=45"}],"version-history":[{"count":1,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/45\/revisions"}],"predecessor-version":[{"id":223,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/45\/revisions\/223"}],"wp:attachment":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=45"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=45"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=45"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}