{"id":44,"date":"2006-09-14T09:14:56","date_gmt":"2006-09-14T00:14:56","guid":{"rendered":"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/?p=44"},"modified":"2014-02-10T09:39:15","modified_gmt":"2014-02-10T00:39:15","slug":"6_loading_buffer","status":"publish","type":"post","link":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/?p=44","title":{"rendered":"6\u00d7 Loading Buffer"},"content":{"rendered":"<div>\n\u6838\u9178\u306e\u30a2\u30ac\u30ed\u30fc\u30b9\u96fb\u6c17\u6cf3\u52d5\u7528\n<\/div>\n<ul>\n<li>10 mg bromophenol blue\u300110 mg xylene cyanol FF\u300110 mg orange G \u306e\u3046\u3061\uff11\u3064\u4ee5\u4e0a<\/li>\n<li>4 g sucrose\u30011.5 g Ficoll 400\u30013 ml glycerol \u306e\u3044\u305a\u308c\u304b\uff11\u3064<\/li>\n<li>H2O \u3092\u52a0\u3048\u3066 10 ml \u306b\u3059\u308b<\/li>\n<\/ul>\n<p><!--more--><\/p>\n<div>\nFicoll\u3084glycerol\u306e\u5834\u5408\u306f\u5ba4\u6e29\u4fdd\u5b58\u3082\u53ef\u3002Sucrose\u306e\u5834\u5408\u306f\u30ab\u30d3\u304c\u751f\u3048\u306a\u3044\u3088\u3046\u51b7\u8535\u4fdd\u5b58\u3002RNA\u306b\u4f7f\u3046\u306a\u3089glycerol\u306b\u3057\u3066\u30aa\u30fc\u30c8\u30af\u30ec\u30fc\u30d6\n<\/div>\n<div>\nBPB\u306f200\u301c300 bp\u4ed8\u8fd1\u3001XC\u306f4 kbp\u4ed8\u8fd1\u3001OG\u306f50 bp\u4ed8\u8fd1\u306b\u4f4d\u7f6e\u3059\u308b\u3002Ethydium bromide\u67d3\u8272\u3057\u305fDNA\u306e\u30d0\u30f3\u30c9\u306bBPB\u3084XC\u304c\u91cd\u306a\u308b\u3068\u86cd\u5149\u304c\u5f31\u307e\u308b\u30022.5 mg\/ml\u3068\u3059\u308b\u30ec\u30b7\u30d4\u304c\u591a\u3044\u304c\u3001\u3058\u3083\u307e\u306b\u306a\u3089\u306a\u3044\u3088\u3046\u8584\u304f\u3057\u305f\u307b\u3046\u304c\u3088\u3044\u3002BPB\u306e\u6fc3\u5ea6\u3092\u4e0b\u3052\u308b\u3068\u3001\u6db2\u304c\u30c1\u30c3\u30d7\u306e\u5148\u306b\u307e\u3068\u308f\u308a\u3064\u304f\u306e\u304c\u6e1b\u308b\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u6838\u9178\u306e\u30a2\u30ac\u30ed\u30fc\u30b9\u96fb\u6c17\u6cf3\u52d5\u7528 10 mg bromophenol blue\u300110 mg xylene cyanol FF\u300110 mg orange G \u306e\u3046\u3061\uff11\u3064\u4ee5\u4e0a 4 g sucrose\u30011.5 g Ficoll 40<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":false,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[3,4],"tags":[],"class_list":["post-44","post","type-post","status-publish","format-standard","hentry","category-buffers-media","category-molecular-biology"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p4fFyn-I","jetpack-related-posts":[],"_links":{"self":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/44","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=44"}],"version-history":[{"count":1,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/44\/revisions"}],"predecessor-version":[{"id":222,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/44\/revisions\/222"}],"wp:attachment":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=44"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=44"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=44"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}