{"id":36,"date":"2007-07-24T16:48:37","date_gmt":"2007-07-24T07:48:37","guid":{"rendered":"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/?p=36"},"modified":"2014-02-10T09:39:14","modified_gmt":"2014-02-10T00:39:14","slug":"nbtbcip","status":"publish","type":"post","link":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/?p=36","title":{"rendered":"NBT\/BCIP\u3092\u5171\u7126\u70b9\u3067\u64ae\u5f71\u3059\u308b"},"content":{"rendered":"<p>NBT\/BCIP\u306f\u8fd1\u8d64\u5916\u306e\u86cd\u5149\u3092\u767a\u3059\u308b\u3002<br \/>\nFV-1000\u3068\u5bfe\u726920x\u3092\u4f7f\u3046\u5834\u5408\uff1a<br \/>\n\u30d4\u30f3\u30db\u30fc\u30eb\uff1a\u81ea\u52d5\u3088\u308a\u6570\u5272\u5927\u304d\u3081<br \/>\n\u30b9\u30ad\u30e3\u30f3\u901f\u5ea6\uff1d10\u301c20 us\/pixel\u3001\u30ab\u30eb\u30de\u30f3\uff1d32\u301c16\u56de<br \/>\n\u52b1\u8d77\uff1a635nm\u300150\u301c100\uff05<br \/>\n\u86cd\u5149\uff1a700\u301c800nm<br \/>\nGain\uff1d5\u3001HV\u3068Offset\u306f\u3001\u30aa\u30fc\u30c8\u3067\u6c7a\u3081\u305f\u3042\u3068\u3001Hi-Low\u8868\u793a\u3067\u304d\u3064\u3081\u306b\u8abf\u7bc0<br \/>\n\u3082\u3068\u306e\u30ce\u30a4\u30ba\u304c\u591a\u304f\u3001\u30b3\u30f3\u30c8\u30e9\u30b9\u30c8\u304c\u4f4e\u3044\u3002\u30b3\u30f3\u30c8\u30e9\u30b9\u30c8\u9ad8\u3081\u306bHV\/Offset\u3092\u304d\u3081\u3001\u30ce\u30a4\u30ba\u304c\u30aa\u30fc\u30d0\u30fc\u30d5\u30ed\u30fc\u3057\u306b\u304f\u3044\u3088\u3046\u3001\u30b9\u30ad\u30e3\u30f3\u9045\u3081\u3067\u30ab\u30eb\u30de\u30f3\u306e\u56de\u6570\u3092\u6e1b\u3089\u3057\u305f\u307b\u3046\u304c\u3088\u3044\u3002<br \/>\n\u81ea\u5bb6\u86cd\u5149\u3082\u5165\u308b\u306e\u3067\u3001\u660e\u8996\u91ce\u3068\u3044\u3063\u3057\u3087\u306b\u64ae\u3063\u305f\u307b\u3046\u304c\u5b89\u5fc3\u3002<br \/>\nTrinh, L.A. et al. (2007). Fluorescent in situ hybridization employing the conventional NBT\/BCIP chromogenic stain. BioTechniques 42: 756-759.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>NBT\/BCIP\u306f\u8fd1\u8d64\u5916\u306e\u86cd\u5149\u3092\u767a\u3059\u308b\u3002 FV-1000\u3068\u5bfe\u726920x\u3092\u4f7f\u3046\u5834\u5408\uff1a \u30d4\u30f3\u30db\u30fc\u30eb\uff1a\u81ea\u52d5\u3088\u308a\u6570\u5272\u5927\u304d\u3081 \u30b9\u30ad\u30e3\u30f3\u901f\u5ea6\uff1d10\u301c20 us\/pixel\u3001\u30ab\u30eb\u30de\u30f3\uff1d32\u301c16\u56de \u52b1\u8d77\uff1a635nm\u300150\u301c100\uff05 \u86cd\u5149<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":false,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[10,9,5],"tags":[],"class_list":["post-36","post","type-post","status-publish","format-standard","hentry","category-cell-biology","category-developmental-biology","category-microscopy"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/s4fFyn-nbtbcip","jetpack-related-posts":[],"_links":{"self":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/36","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=36"}],"version-history":[{"count":1,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/36\/revisions"}],"predecessor-version":[{"id":211,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/36\/revisions\/211"}],"wp:attachment":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=36"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=36"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=36"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}