{"id":336,"date":"2014-11-14T12:30:54","date_gmt":"2014-11-14T03:30:54","guid":{"rendered":"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/?p=336"},"modified":"2016-04-17T09:53:44","modified_gmt":"2016-04-17T00:53:44","slug":"kod-fx-neo","status":"publish","type":"post","link":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/?p=336","title":{"rendered":"KOD FX Neo"},"content":{"rendered":"<p><a href=\"http:\/\/www.toyobo.co.jp\/seihin\/xr\/lifescience\/products\/product\/pcr\/archives\/2010\/10\/kod_fx_neo.html\" target=\"_blank\">KOD FX Neo<\/a>\u306f\u4e0b\u306e\u7279\u5fb4\u3092\u5099\u3048\u305fPCR\u7528\u9175\u7d20\u3002Genotyping\u00a0\u306b\u4f7f\u7528\u4e2d\u3002<!--more--><\/p>\n<ul>\n<li>\u96e3\u914d\u5217\u306e\u5897\u5e45<\/li>\n<li>\u30af\u30eb\u30fc\u30c9\u30e9\u30a4\u30bb\u30fc\u30c8\u304b\u3089\u306e\u5897\u5e45<\/li>\n<li>\u5fae\u751f\u7269\u30fb\u751f\u4f53\u6210\u5206\u7b49\u304b\u3089\u306e\u76f4\u63a5PCR<\/li>\n<\/ul>\n<p>\u6587\u66f8<\/p>\n<ul>\n<li><a href=\"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/wp-content\/uploads\/2014\/11\/20140619KFX-2_Flyer.pdf\">\u30d1\u30f3\u30d5\u30ec\u30c3\u30c8<\/a><\/li>\n<li><a href=\"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/wp-content\/uploads\/2014\/11\/KFX-201.pdf\">\u30de\u30cb\u30e5\u30a2\u30eb<\/a><\/li>\n<\/ul>\n<p>\u65b9\u6cd5<\/p>\n<p>x \u00b5l H2O<br \/>\n25 \u00b5l 2\u00d7Buffer<br \/>\n10 \u00b5l 2 mM dNTPs<br \/>\n0.75\u20131.5 \u00b5l 2 mM Primer\u00a0mix<br \/>\nY \u00b5l template<br \/>\n1 \u00b5l KOD FX Neo<br \/>\n\u2013\u2013\u2013\u2013\u2013\u2013\u2013\u2013\u2013\u2013\u2013<br \/>\n50\u00b5l total<\/p>\n<p>2 m @ 94\u00ba<br \/>\n|<br \/>\n10s @ 98\u00ba<br \/>\n60s\/kb @68\u00ba, 25\u201345x<\/p>\n<p>2 m @ 94\u00ba<br \/>\n|<br \/>\n10s @ 98\u00ba<br \/>\n30s @ Tm<br \/>\n60s\/kb @68\u00ba, 25\u201345x<\/p>\n","protected":false},"excerpt":{"rendered":"<p>KOD FX Neo\u306f\u4e0b\u306e\u7279\u5fb4\u3092\u5099\u3048\u305fPCR\u7528\u9175\u7d20\u3002Genotyping\u00a0\u306b\u4f7f\u7528\u4e2d\u3002<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[4],"tags":[19],"class_list":["post-336","post","type-post","status-publish","format-standard","hentry","category-molecular-biology","tag-pcr"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p4fFyn-5q","jetpack-related-posts":[],"_links":{"self":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/336","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=336"}],"version-history":[{"count":4,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/336\/revisions"}],"predecessor-version":[{"id":342,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/336\/revisions\/342"}],"wp:attachment":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=336"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=336"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=336"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}