{"id":29,"date":"2006-09-25T22:50:29","date_gmt":"2006-09-25T13:50:29","guid":{"rendered":"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/?p=29"},"modified":"2014-02-10T09:39:14","modified_gmt":"2014-02-10T00:39:14","slug":"in_situclearing","status":"publish","type":"post","link":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/?p=29","title":{"rendered":"in situ\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30bc\u30fc\u30b7\u30e7\u30f3\u3067\u67d3\u8272\u3057\u305f\u80da\u306eClearing\u6cd5"},"content":{"rendered":"
\nClearing\u3068\u306f\u3001\u9855\u5fae\u93e1\u89b3\u5bdf\u306e\u305f\u3081\u306b\u6a19\u672c\u3092\u900f\u660e\u306b\u3059\u308b\u51e6\u7406\u306e\u3053\u3068\u3002\u6a19\u672c\u3068\u5c48\u6298\u7387\u306e\u8fd1\u3044\u6eb6\u5a92\u306b\u6a19\u672c\u3092\u6d78\u6f2c\u3057\u3001\u754c\u9762\u3067\u306e\u5149\u306e\u6563\u4e71\u3092\u4f4e\u6e1b\u3055\u305b\u308b\u3002in situ\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30bc\u30fc\u30b7\u30e7\u30f3\u306e\u5834\u5408\u3001\u672a\u53cd\u5fdc\u306e\u57fa\u8cea\u3092\u6eb6\u304b\u3057\u3066\u67d3\u8272\u306e\u30b3\u30f3\u30c8\u30e9\u30b9\u30c8\u3092\u9ad8\u3081\u308b\u52b9\u679c\u3082\u3042\u308b\u3002\n<\/div>\n

\u30b0\u30ea\u30bb\u30ea\u30f3<\/h3>\n
\nClearing\u52b9\u679c\u306f\u5f31\u3044\u304c\u6c34\u6027\u3067\u6271\u3044\u3084\u3059\u3044\u3002PBS\u306a\u3069\u306750\u301c100\uff05\u5f31\u306b\u3057\u3066\u7528\u3044\u308b\u3002\u6d78\u900f\u5727\u3067\u53ce\u7e2e\u3057\u306a\u3044\u3088\u3046\u3001\u6570\u6bb5\u968e\u306b\u308f\u3051\u3066\u6fc3\u5ea6\u3092\u4e0a\u3052\u308b\u3002\u80da\u304c\u6c88\u3080\u306e\u3092\u898b\u8a08\u3089\u3063\u3066\u6b21\u306e\u6bb5\u968e\u306e\u6db2\u306b\u79fb\u3059\u3002\n<\/div>\n

Murray’s solution<\/h3>\n
\n\u30d9\u30f3\u30b8\u30eb\u30a2\u30eb\u30b3\u30fc\u30eb\uff08benzyl alcohol\uff09\u3068\u5b89\u606f\u9999\u9178\u30d9\u30f3\u30b8\u30eb\uff08benzyl benzoate\uff09\u306e1:2\u6df7\u5408\u6db2\u3002\u5375\u9ec4\u306e\u5c48\u6298\u7387\u306b\u8fd1\u3044\u5c48\u6298\u7387\uff081.56\uff09\u3092\u6301\u3064\u305f\u3081\u306bclearing\u52b9\u679c\u304c\u5f37\u304f\u3001\u80da\u306f\u307b\u3068\u3093\u3069\u900f\u660e\u306b\u306a\u308b\u3002\u6b20\u70b9\u306f\u3001NBT\/BCIP\u306e\u9752\u3044\u67d3\u8272\u3092\u5c11\u3057\u305a\u3064\u6eb6\u304b\u3059\u3053\u3068\u3001\u30d7\u30e9\u30b9\u30c1\u30c3\u30af\u3092\u4fb5\u3059\u3053\u3068\u3001\u63ee\u767a\u81ed\u304c\u3042\u308b\u3053\u3068\u3002\u6c34\u6eb6\u6db2\u4e2d\u306e\u80da\u3092\u6b21\u306e\u3088\u3046\u306b\u7f6e\u63db\u3059\u308b\n<\/div>\n
    \n
  1. 100%\u30e1\u30bf\u30ce\u30fc\u30eb\u300010\u5206\u9593\u00d72<\/li>\n
  2. Murray’s solution\u306b\u79fb\u3059<\/li>\n<\/ol>\n
    \nhttp:\/\/zfin.org\/zf_info\/monitor\/vol2.1\/vol2.1.html#WHOLE-MOUNT%20IN%20SITU%20HYBRIDIZATIONS%20IN<\/a>\n<\/div>\n

    \u30c6\u30c8\u30e9\u30d2\u30c9\u30ed\u30ca\u30d5\u30bf\u30ec\u30f3\uff08tetrahydronaphtalene\uff09<\/h3>\n
    \nMurray’s solution\u3068\u540c\u7b49\u306eclearing\u52b9\u679c\u304c\u3042\u308b\u304c\u3001\u9752\u3044\u67d3\u8272\u306e\u878d\u89e3\u306f\u5c11\u306a\u3044\u3002
    \n\u6c34\u6eb6\u6db2\u4e2d\u306e\u80da\u3092\u6b21\u306e\u3088\u3046\u306b\u7f6e\u63db\u3059\u308b\uff1a\n<\/div>\n
      \n
    1. 100%\u30e1\u30bf\u30ce\u30fc\u30eb\u30005\u5206\u9593<\/li>\n
    2. \u30a4\u30bd\u30d7\u30ed\u30d1\u30ce\u30fc\u30eb\u300010\u5206\u9593<\/li>\n
    3. \u30c6\u30c8\u30e9\u30d2\u30c9\u30ed\u30ca\u30d5\u30bf\u30ec\u30f3\u300010\u5206\u9593<\/li>\n<\/ol>\n
      \n\u9752\u3044\u67d3\u8272\u3092\u3086\u3063\u304f\u308a\u3068\u6eb6\u304b\u3059\u306e\u3067\u3001\u9577\u671f\u4fdd\u5b58\u306b\u306f\u5411\u304b\u306a\u3044\u3002\u3044\u3063\u305f\u3093PCR\u7528\u306e\u30df\u30cd\u30e9\u30eb\u30aa\u30a4\u30eb\u306b\u79fb\u3057\u3066\u4fdd\u5b58\u3057\u3066\u304a\u304d\u3001\u89b3\u5bdf\u6642\u306b\u30c6\u30c8\u30e9\u30d2\u30c9\u30ed\u30ca\u30d5\u30bf\u30ec\u30f3\u306b\u623b\u3059\n<\/div>\n
      \nhttp:\/\/zfin.org\/zf_info\/monitor\/vol2.1\/vol2.1.html#TETRAHYDRONAPHTHALENE:%20A%20SMELLY%20BUT<\/a>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

      Clearing\u3068\u306f\u3001\u9855\u5fae\u93e1\u89b3\u5bdf\u306e\u305f\u3081\u306b\u6a19\u672c\u3092\u900f\u660e\u306b\u3059\u308b\u51e6\u7406\u306e\u3053\u3068\u3002\u6a19\u672c\u3068\u5c48\u6298\u7387\u306e\u8fd1\u3044\u6eb6\u5a92\u306b\u6a19\u672c\u3092\u6d78\u6f2c\u3057\u3001\u754c\u9762\u3067\u306e\u5149\u306e\u6563\u4e71\u3092\u4f4e\u6e1b\u3055\u305b\u308b\u3002in situ\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30bc\u30fc\u30b7\u30e7\u30f3\u306e\u5834\u5408\u3001\u672a\u53cd\u5fdc\u306e\u57fa\u8cea\u3092\u6eb6\u304b\u3057\u3066\u67d3\u8272\u306e\u30b3\u30f3\u30c8\u30e9\u30b9\u30c8<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":false,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[3,9],"tags":[],"class_list":["post-29","post","type-post","status-publish","format-standard","hentry","category-buffers-media","category-developmental-biology"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p4fFyn-t","jetpack-related-posts":[],"_links":{"self":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/29","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=29"}],"version-history":[{"count":1,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/29\/revisions"}],"predecessor-version":[{"id":217,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/29\/revisions\/217"}],"wp:attachment":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=29"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=29"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=29"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}