{"id":28,"date":"2006-09-25T23:35:04","date_gmt":"2006-09-25T14:35:04","guid":{"rendered":"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/?p=28"},"modified":"2014-02-10T09:39:14","modified_gmt":"2014-02-10T00:39:14","slug":"sybr_gold","status":"publish","type":"post","link":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/?p=28","title":{"rendered":"SYBR Gold"},"content":{"rendered":"<div>\nDNA\u3068RNA\u3092\u67d3\u3081\u308b\u3002\u9ad8\u611f\u5ea6\u3002UV\u307e\u305f\u306f\u9752\u3044\u30c8\u30e9\u30f3\u30b9\u30a4\u30eb\u30df\u30cd\u30fc\u30bf\u30fc\u3067\u53ef\u8996\u5316\uff08\u5438\u53ce\uff1d300 nm\u3068495 nm\u3001\u86cd\u5149\uff1d537 nm\uff09\u3002\u6cf3\u52d5\u5f8c\u306b\u30b2\u30eb\u3092\u67d3\u3081\u308b\uff08\u30b2\u30eb\u3084\u30b5\u30f3\u30d7\u30eb\u306b\u6df7\u305c\u308b\u3068\u6cf3\u52d5\u304c\u4e71\u308c\u308b\uff09\n<\/div>\n<h3>\u30b9\u30c8\u30c3\u30af<\/h3>\n<div>\n10,000\u00d7DMSO\u6eb6\u6db2\u300220\u2103\u4ee5\u4e0b\u3067\u906e\u5149\u4fdd\u5b58\u3002\u534a\u5e74\u304b\u3089\u4e00\u5e74\u9593\u5b89\u5b9a\u3002\u5206\u6ce8\u3057\u3066\u4fdd\u5b58\n<\/div>\n<h3>\u4f7f\u7528\u6cd5<\/h3>\n<ol>\n<li>DMSO\u30b9\u30c8\u30c3\u30af\u3092\u5ba4\u6e29\u306b\u3057\u3001\u9060\u5fc3\u3057\u3066\u6eb6\u6db2\u3092\u30c1\u30e5\u30fc\u30d6\u306e\u5e95\u306b\u96c6\u3081\u308b<\/li>\n<li>5 \u03bcl\u306e\u30b9\u30c8\u30c3\u30af\u309250 ml\u306eTE\u3001TBE\u307e\u305f\u306fTAE\u3067\u5e0c\u91c8\u3059\u308b\u3002pH7\u301c8.5\u3067\u3042\u308b\u3053\u3068\u30024\u2103\u3067\u6570\u9031\u9593\u4fdd\u5b58\u53ef\u80fd\u30023\u301c4\u56de\u518d\u4f7f\u7528\u3067\u304d\u308b\u3002\u30ac\u30e9\u30b9\u5bb9\u5668\u306f\u4e0d\u9069<\/li>\n<li>\u30b2\u30eb\u3092\u4f7f\u7528\u6db2\u306b\u6d78\u6f2c\u3057\u300110\u301c40\u5206\u9593\u64b9\u62cc<\/li>\n<\/ol>\n<div>\n\u30ed\u30fc\u30c9\u3059\u308bDNA\u91cf\u306f\u30a8\u30c1\u30b8\u30a6\u30e0\u30d6\u30ed\u30de\u30a4\u30c9\u306e1\/10\u304f\u3089\u3044\u306b\u3059\u308b\u3002\u30b2\u30eb\u306eDNA\u306e\u6fc3\u3044\u90e8\u5206\u306b\u306f\u8272\u7d20\u304c\u6d78\u900f\u3057\u306b\u304f\u3044\u306e\u3067\u3001\u76f8\u5bfe\u7684\u306b\u30de\u30a4\u30ca\u30fc\u306a\u30d0\u30f3\u30c9\u304c\u3088\u308a\u5f37\u304f\u67d3\u307e\u308b\u3002\u5909\u306b\u601d\u3048\u305f\u3089\u30b2\u30eb\u3092\u30b9\u30e9\u30a4\u30b9\u3057\u3066\u65ad\u9762\u3067\u8272\u7d20\u306e\u6d78\u900f\u3092\u78ba\u8a8d\u3059\u308b\u3002\n<\/div>\n<ul>\n<li><a href=\"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/td004.pdf\">\u30de\u30cb\u30e5\u30a2\u30eb<\/a><\/li>\n<li><a href=\"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/mp11494.pdf\">Tips<\/a><\/li>\n<li><a href=\"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/120.pdf\">\u30ab\u30bf\u30ed\u30b0<\/a><\/li>\n<li><a href=\"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/s11494001.pdf\">EtBr\u3068\u306e\u6bd4\u8f03<\/a><\/li>\n<li><a href=\"http:\/\/www.ikushimo.com\/news\/2006\/03\/04.html\">\u611f\u52d5\u3057\u305f\u4eba<\/a><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>DNA\u3068RNA\u3092\u67d3\u3081\u308b\u3002\u9ad8\u611f\u5ea6\u3002UV\u307e\u305f\u306f\u9752\u3044\u30c8\u30e9\u30f3\u30b9\u30a4\u30eb\u30df\u30cd\u30fc\u30bf\u30fc\u3067\u53ef\u8996\u5316\uff08\u5438\u53ce\uff1d300 nm\u3068495 nm\u3001\u86cd\u5149\uff1d537 nm\uff09\u3002\u6cf3\u52d5\u5f8c\u306b\u30b2\u30eb\u3092\u67d3\u3081\u308b\uff08\u30b2\u30eb\u3084\u30b5\u30f3\u30d7\u30eb\u306b\u6df7\u305c\u308b\u3068\u6cf3\u52d5\u304c\u4e71\u308c\u308b\uff09 \u30b9\u30c8\u30c3\u30af 10,000\u00d7<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":false,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[4],"tags":[],"class_list":["post-28","post","type-post","status-publish","format-standard","hentry","category-molecular-biology"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p4fFyn-s","jetpack-related-posts":[],"_links":{"self":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/28","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=28"}],"version-history":[{"count":1,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/28\/revisions"}],"predecessor-version":[{"id":216,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/28\/revisions\/216"}],"wp:attachment":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=28"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=28"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=28"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}