{"id":27,"date":"2006-09-27T17:18:50","date_gmt":"2006-09-27T08:18:50","guid":{"rendered":"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/?p=27"},"modified":"2014-02-10T09:39:14","modified_gmt":"2014-02-10T00:39:14","slug":"infusion_drydown_pcr_cloning_k","status":"publish","type":"post","link":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/?p=27","title":{"rendered":"In-Fusion Dry-Down PCR Cloning Kit"},"content":{"rendered":"<p>\u30a4\u30f3\u30b5\u30fc\u30c8\uff1a\u30d9\u30af\u30bf\u30fc = 2 : 1\u306b\u306a\u308b\u3088\u3046\u306b\u52a0\u3048\u308b\u30a4\u30f3\u30b5\u30fc\u30c8\u306e\u91cf\uff081 kb\u3042\u305f\u308a\uff09\uff1a<br \/>\n100 ng pDNR-Dual + 41 ng\/kb \u30a4\u30f3\u30b5\u30fc\u30c8<br \/>\n100 ng pDNR-CMV + 36 ng\/kb \u30a4\u30f3\u30b5\u30fc\u30c8<br \/>\n<a href=\"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/PT3754-1.pdf\">\u30de\u30cb\u30e5\u30a2\u30eb<\/a><br \/>\n<a href=\"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/PT3754-2.pdf\">Protocol-at-a-Glance<\/a><br \/>\n<a href=\"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/PT3754-4.pdf\">\u88dc\u8db3<\/a><br \/>\n<a href=\"http:\/\/www.clontech.com\/clontech\/infusion\/index.shtml\">\u88fd\u54c1\u30da\u30fc\u30b8\uff08\u82f1\u8a9e\uff09<\/a><br \/>\n<a href=\"http:\/\/clontech.takara-bio.co.jp\/product\/catalog\/2003088.shtml\">\u88fd\u54c1\u30da\u30fc\u30b8\uff08\u30bf\u30ab\u30e9\u30d0\u30a4\u30aa\uff09<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u30a4\u30f3\u30b5\u30fc\u30c8\uff1a\u30d9\u30af\u30bf\u30fc = 2 : 1\u306b\u306a\u308b\u3088\u3046\u306b\u52a0\u3048\u308b\u30a4\u30f3\u30b5\u30fc\u30c8\u306e\u91cf\uff081 kb\u3042\u305f\u308a\uff09\uff1a 100 ng pDNR-Dual + 41 ng\/kb \u30a4\u30f3\u30b5\u30fc\u30c8 100 ng pDNR-CMV + 36 ng\/kb \u30a4\u30f3\u30b5\u30fc<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":false,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[4],"tags":[],"class_list":["post-27","post","type-post","status-publish","format-standard","hentry","category-molecular-biology"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p4fFyn-r","jetpack-related-posts":[],"_links":{"self":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/27","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=27"}],"version-history":[{"count":1,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/27\/revisions"}],"predecessor-version":[{"id":215,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/27\/revisions\/215"}],"wp:attachment":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=27"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=27"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=27"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}