{"id":18,"date":"2006-08-24T14:59:53","date_gmt":"2006-08-24T05:59:53","guid":{"rendered":"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/?p=18"},"modified":"2014-02-10T09:37:56","modified_gmt":"2014-02-10T00:37:56","slug":"zero_blunt_topo_pcr_cloning_ki","status":"publish","type":"post","link":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/?p=18","title":{"rendered":"Zero Blunt TOPO PCR Cloning Kit, Invitrogen"},"content":{"rendered":"<p>KOD\u306a\u3069\u306ePCR\u9175\u7d20\u3067\u3067\u304d\u308b\u5e73\u6ed1\u672b\u7aef\u306e\u5897\u6b96\u7523\u7269\u3092\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3059\u308b\u305f\u3081\u306e\u30ad\u30c3\u30c8\u3002\u30a4\u30f3\u30b5\u30fc\u30c8\u304c\u30d9\u30af\u30bf\u30fc\u306b\u5165\u3089\u306a\u3044\u3068\u5927\u8178\u83cc\u304c\u81f4\u6b7b\u306b\u306a\u308b\u306e\u3067\u3001\u30b9\u30ab\u304c\u5c11\u306a\u3044\uff08\u3053\u3068\u304c\u591a\u3044\uff09\u30023Kb\u3092\u8d85\u3048\u308b\u30a4\u30f3\u30b5\u30fc\u30c8\u3067\u306f\u52b9\u7387\u304c\u8457\u3057\u304f\u4e0b\u304c\u308b\u306e\u3067\u3001\u307b\u304b\u306e\u30ad\u30c3\u30c8\u3092\u5f53\u305f\u3063\u305f\u307b\u3046\u304c\u3088\u3044\u3002<strong>\u30ab\u30ca\u30de\u30a4\u30b7\u30f3<\/strong>\u3067\u9078\u629e\u3002<br \/>\n<a href=\"http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/pcrbluntiitopo_map.html\" onclick=\"window.open('http:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/pcrbluntiitopo_map.html','popup','width=950,height=1002,scrollbars=no,resizable=no,toolbar=no,directories=no,location=no,menubar=no,status=no,left=0,top=0'); 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