50 ml\u30c1\u30e5\u30fc\u30d6\u306b\u6b21\u3092\u52a0\u3048\u3066\u6df7\u548c
\n6 ml glycerol
\n12 ml 0.2 M Tris HCl, pH 9.5
\n\uff08\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u306e\u81f3\u6ef4pH\u3002\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u3067\u306a\u3051\u308c\u3070\u4ed6\u306epH\u3067\u3082\u3088\u3044\uff09
\n2.4 g Mowiol\u3092\u52a0\u3048\u3001\u3059\u3050\u306b\u64b9\u62cc\u3057\u3066Mowiol\u3092\u5206\u6563\u3055\u305b\u308b\uff08\u6025\u304c\u306a\u3044\u3068\u304b\u305f\u307e\u308a\u304c\u3067\u304d\u3066\u6eb6\u3051\u306b\u304f\u304f\u306a\u308b\uff09
\n\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30bc\u30fc\u30b7\u30e7\u30f3\u7528\u30aa\u30fc\u30d6\u30f3\u3067\u64b9\u62cc\u3057\u306a\u304c\u308950\u2103\u3067\u52a0\u71b1\uff08\u306a\u304b\u306a\u304b\u6eb6\u3051\u306a\u3044\uff09
\nDABCO\u3092\uff082.5\u301c\uff095%\u306b\u306a\u308b\u3088\u3046\u52a0\u3048\u308b
\nH2O\u3092\u52a0\u3048\u306624 ml\u3068\u3059\u308b
\n\u9060\u5fc3\u3057\u3066\u4e0d\u53ef\u6eb6\u7269\u3092\u9664\u304f
\n\u30d0\u30a4\u30a2\u30eb\u3067\u5c0f\u5206\u3051\u3057\u3066\u51b7\u8535\u4fdd\u5b58
\n\u767d\u304f\u56fa\u307e\u3063\u305f\u3089\u3001\u96fb\u5b50\u30ec\u30f3\u30b8\u3067\u6570\u79d2\u6e29\u3081\u308b\u3068\u6eb6\u3051\u308b
\n\u4f7f\u7528\u6cd5\uff1a
\n\u30b9\u30e9\u30a4\u30c9\u30ac\u30e9\u30b9\u4e0a\u306e\u5207\u7247\u306b50\u301c100 \u03bcl\u306e\u5c01\u5165\u5264\u3092\u30de\u30a4\u30af\u30ed\u30d4\u30da\u30c3\u30c8\u3067\u304a\u304f
\n\u30ab\u30d0\u30fc\u30ac\u30e9\u30b9\u3092\u304b\u3051\u308b
\n\u7e01\u306b\u306f\u307f\u51fa\u305f\u5c01\u5165\u5264\u3092\u30ad\u30e0\u30ef\u30a4\u30d7\u306a\u3069\u3067\u5438\u3044\u53d6\u308b
\n\u30ab\u30d0\u30fc\u30ac\u30e9\u30b9\u306e\u89d2\u3092\u30de\u30cb\u30ad\u30e5\u30a2\u3067\u4eee\u6b62\u3081
\n\u4e00\u6669\u51b7\u8535\u5eab\u306b\u7f6e\u304f\u3068\u5c01\u5165\u5264\u304c\u4e7e\u3044\u3066\u56fa\u307e\u308b
\n\u56fa\u307e\u3089\u306a\u3044\u3046\u3061\u306f\u9000\u8272\u9632\u6b62\u52b9\u679c\u304c\u3067\u306a\u3044<\/p>\n","protected":false},"excerpt":{"rendered":"
50 ml\u30c1\u30e5\u30fc\u30d6\u306b\u6b21\u3092\u52a0\u3048\u3066\u6df7\u548c 6 ml glycerol 12 ml 0.2 M Tris HCl, pH 9.5 \uff08\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u306e\u81f3\u6ef4pH\u3002\u30d5\u30eb\u30aa\u30ec\u30bb\u30a4\u30f3\u3067\u306a\u3051\u308c\u3070\u4ed6\u306epH\u3067\u3082\u3088\u3044\uff09 2.4 g Mowiol\u3092<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":false,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[5],"tags":[],"class_list":["post-14","post","type-post","status-publish","format-standard","hentry","category-microscopy"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/s4fFyn-post_5","jetpack-related-posts":[],"_links":{"self":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/14","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=14"}],"version-history":[{"count":1,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/14\/revisions"}],"predecessor-version":[{"id":241,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=\/wp\/v2\/posts\/14\/revisions\/241"}],"wp:attachment":[{"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=14"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=14"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/anatomy.med.gunma-u.ac.jp\/protocols\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=14"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}